| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.047055 |
| Chromosome: | chromosome 5 |
| Location: | 3429732 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g233304 | CHI3 | (1 of 2) K01183 - chitinase (E3.2.1.14); Chitinase-like hydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCTGATGCCCCGCATGTGCCAGGCCAATGCCAATACCCTGGAGACTGC |
| Internal bar code: | GCTCTGGTCTAGATCTGTGTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 920 |
| LEAP-Seq percent confirming: | 91.6667 |
| LEAP-Seq n confirming: | 22 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATAGGATCTCCCACTGCC |
| Suggested primer 2: | GGCCTAGTGCAATGGGTGAT |