| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.047067 |
| Chromosome: | chromosome 12 |
| Location: | 5649494 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g531700 | ADA,ADA3 | Adenosine/AMP deaminase; (1 of 1) 3.5.4.6 - AMP deaminase / Myoadenylate deaminase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATCGCAGGATGCGGTGCATGTCGGTGAAGAACTCGGCGGCCGTGCCCGG |
| Internal bar code: | GAGGCCGTCCCGAAAGTCAGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 724 |
| LEAP-Seq percent confirming: | 70.5882 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCGCGGCTTTAACAACAAC |
| Suggested primer 2: | GAACACCTCCTTCAGCGTCA |