Insertion junction: CLIP2.047089_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.047089
Chromosome:chromosome 2
Location:3948987
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g102300 (1 of 1) PTHR31013 - THAUMATIN FAMILY PROTEIN-RELATED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCGTACAACGTACATCGTAACAATGGTGCTACTGTCAATGCGCCTTCCA
Internal bar code:GAGTGACACGCGTCGGGATCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:4148
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:92
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:92

Suggested primers for confirmation by PCR

Suggested primer 1:GTCATCCCTACCCTACCCGT
Suggested primer 2:CGCTGGGGTTGACTGATTCT