Insertion junction: CLIP2.047101_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.047101
Chromosome:chromosome 4
Location:2850245
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g224250 FAP147 Flagellar Associated Protein 147; (1 of 1) PF14646 - MYCBP-associated protein family (MYCBPAP) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCAAACCGCGCACGCTGCTGTACTCCCACCCTCCCCCCCACCGCTGCGC
Internal bar code:AAATGAGTGCATCGGAATAGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:73
LEAP-Seq percent confirming:11.1111
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:CACACACACACACACACACC
Suggested primer 2:GGCACCAGCAATGCGAATAG