| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.047103 |
| Chromosome: | chromosome 6 |
| Location: | 5493311 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g286350 | UMM6 | UbiE/COQ5 methyltransferase family protein; (1 of 2) PTHR10108//PTHR10108:SF830 - METHYLTRANSFERASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCGCCCTCTATCTCCAATCCGCGACCGCCCTGGCTCCTGCCTCGGCTG |
| Internal bar code: | TTCGTCCCCAAGGGCCCAGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3169 |
| LEAP-Seq percent confirming: | 87.8378 |
| LEAP-Seq n confirming: | 65 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 74 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGTCTCCCACCGAGGTAGT |
| Suggested primer 2: | AGCACCACCGCATTGTATCA |