| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.047106 |
| Chromosome: | chromosome 1 |
| Location: | 6038190 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g042650 | (1 of 17) PF13202 - EF hand (EF-hand_5) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGACTGTATCTTCGGACGGCAAGCTGGTGTCTCGCACCTCCAACAGCACC |
| Internal bar code: | ATTGCGTTGTTTCCGAAGGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2426 |
| LEAP-Seq percent confirming: | 55.5556 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTCAGGGCACTTCATGACA |
| Suggested primer 2: | TGCTGTGTGATTGTTGCGTG |