| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.047110 |
| Chromosome: | chromosome 6 |
| Location: | 2523177 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g269350 | (1 of 1) K13211 - GC-rich sequence DNA-binding factor (GCFC) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCCAGTGCAGTCTTTAGTGCATCCTGTACTCGCTTTTCCACCCACCCA |
| Internal bar code: | CTCCTGGCCCTATCTAATATGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 54 |
| LEAP-Seq percent confirming: | 11.1111 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGTGCACAGGTTTTGCTC |
| Suggested primer 2: | GTGCTCCAAAAACACCACCG |