| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.047117 |
| Chromosome: | chromosome 11 |
| Location: | 3368207 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g476900 | (1 of 1) PTHR12465//PTHR12465:SF0 - UBIQUITIN SPECIFIC PROTEASE HOMOLOG 49 // MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 20 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAGCAACTCGTGTCAACGGCGCTAGAACAGCAGTGCGCAGAGCCGAAGC |
| Internal bar code: | CTAGGGTTAGCGTTCGGACCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 880 |
| LEAP-Seq percent confirming: | 84.2105 |
| LEAP-Seq n confirming: | 32 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGACGTTTTCCTTGTGCTT |
| Suggested primer 2: | ACACCACTCGGCGAATTCAT |