| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.047131 |
| Chromosome: | chromosome 15 |
| Location: | 341368 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g635400 | ZYS3,ZYS3-1 | (1 of 3) PTHR24193//PTHR24193:SF87 - ANKYRIN REPEAT PROTEIN // SUBFAMILY NOT NAMED; Zygote-specific protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCATGACATGAGTTCTGCACATCACAGCCCTCACAGCCCGACACGGCCTG |
| Internal bar code: | TTGGTCTCGCAGATGCGATGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2098 |
| LEAP-Seq percent confirming: | 75.5396 |
| LEAP-Seq n confirming: | 105 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 139 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGGGGTTGACCGTGTGTTT |
| Suggested primer 2: | CTGGCATGTCAGGATGTCGT |