| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.047140 |
| Chromosome: | chromosome 2 |
| Location: | 4354390 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g105287 | (1 of 1) PF13821 - Domain of unknown function (DUF4187) (DUF4187) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGCCTCGCGACGCGACAGGCGTCCCCGAAGAACCCGGCGTTACCAGGTG |
| Internal bar code: | CGGAATCGGACTTCAGTTTGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2475 |
| LEAP-Seq percent confirming: | 92.1053 |
| LEAP-Seq n confirming: | 35 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGACACGGTAAAGCGACAA |
| Suggested primer 2: | GCTGAAGAAGATGGGCTGGT |