Insertion junction: CLIP2.047142_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.047142
Chromosome:chromosome 1
Location:3454417
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g022450 (1 of 3) IPR006502//IPR011705 - Protein of unknown function PDDEXK-like // BTB/Kelch-associated CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAAGGCGCTGGTCGCGCACTTCACCAGCGCGCCCACAACGCTCAACTCAG
Internal bar code:GTTAGCTTAGGGTGGATCGGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:2943
LEAP-Seq percent confirming:98.5507
LEAP-Seq n confirming:68
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:69

Suggested primers for confirmation by PCR

Suggested primer 1:CAAACACCTCCAACTTGGCG
Suggested primer 2:AAGCTTGTCTTTGGCGCAAG