Insertion junction: CLIP2.047150_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.047150
Chromosome:chromosome 15
Location:642398
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre15.g636896 (1 of 1) PTHR11082//PTHR11082:SF29 - TRNA-DIHYDROURIDINE SYNTHASE // TRNA-DIHYDROURIDINE SYNTHASE intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACACCCACACCCACACCCACACCCACACCCACACCCACACCCACACCCA
Internal bar code:TAGGTCGTTGGTCGCGTGACTG

Confirmation - LEAP-Seq

LEAP-Seq distance:1134
LEAP-Seq percent confirming:19.4444
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:29
LEAP-Seq n unique pos:36

Suggested primers for confirmation by PCR

Suggested primer 1:TCATCAACCTGCGTTACCCC
Suggested primer 2:GGAGGGGAGGGGGTAATTCT