Insertion junction: CLIP2.047151_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.047151
Chromosome:chromosome 17
Location:6470309
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g744297 (1 of 1) IPR005181//IPR013830 - Acetylesterase, domain of unknown function DUF303 // SGNH hydrolase-type esterase domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGCACCCTGCCTGCTGCTAGCACTCCCACACTACAGCCAGCCTTCATCC
Internal bar code:CGGTCTAATTCCCTAACGTTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:104
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:AACCTCTTCCATGACTGGCG
Suggested primer 2:CAACAACAGCACCCACCATG