| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.047176 |
| Chromosome: | chromosome 3 |
| Location: | 1220885 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g149600 | CYG53 | (1 of 3) PTHR11920//PTHR11920:SF328 - ADENYLATE AND GUANYLATE CYCLASES // SUBFAMILY NOT NAMED; Adenylate/guanylate cyclase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGACCTGCCGGAGGCGCCCACGCCCGATAAGTTCTTATTCGCGAGGAAGG |
| Internal bar code: | TCGTATAAGCCACGTACCATTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 435 |
| LEAP-Seq percent confirming: | 3.125 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCCAACAGCACAGGATCAT |
| Suggested primer 2: | CAGCACTCATCGTCCTCCTC |