| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.047177 |
| Chromosome: | chromosome 8 |
| Location: | 1370708 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g364100 | (1 of 1) PF13449 - Esterase-like activity of phytase (Phytase-like) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCTCCTCGTTGCGCAGCACGAACGGCACCTTGCGGTCCTTGTCGTGCAG |
| Internal bar code: | TATTCTAACCGTCTGCTGGGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1316 |
| LEAP-Seq percent confirming: | 85.7143 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCACATCCCTTGCGCTTCAT |
| Suggested primer 2: | CATGTGTGTCGCAACAAGCA |