| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.047179 |
| Chromosome: | chromosome 17 |
| Location: | 5784337 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g741200 | EB1,EBP1 | (1 of 1) K10436 - microtubule-associated protein, RP/EB family (MAPRE); Microtubule plus-end binding protein EB1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCACGTCGCGCAGCTTGCTGTAGTAGAAGTCCTTCTCCTTCTCGTAGC |
| Internal bar code: | GAGGCGACCTGACCATTGATTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 405 |
| LEAP-Seq percent confirming: | 8.33333 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAGGGGTGGGCTTGTTTTT |
| Suggested primer 2: | CAGAGGTGCATATCCGGTCC |