Insertion junction: CLIP2.047181_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.047181
Chromosome:chromosome 13
Location:454934
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g564900 MRP3 (1 of 18) 3.6.3.44 - Xenobiotic-transporting ATPase / Steroid-transporting ATPase; ABC transporter, multidrug resistance associated protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGGAATAAACATAGCCACTTCACGGTTAGCAACGAGCGACTTCCATGCA
Internal bar code:TGATTGAATTAAGAATGTGGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:4806
LEAP-Seq percent confirming:97.4359
LEAP-Seq n confirming:38
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:39

Suggested primers for confirmation by PCR

Suggested primer 1:GCTTCGCAAGTGAGTCAAGC
Suggested primer 2:CACGCTGTACAAAGATCGCG