| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.047184 |
| Chromosome: | chromosome 2 |
| Location: | 6274265 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g387350 | CYG30 | (1 of 11) 4.6.1.1//4.6.1.2 - Adenylate cyclase / ATP pyrophosphate-lyase // Guanylate cyclase / Guanylyl cyclase; Adenylate/guanylate cyclase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGCAGGCCGTTCACGTCTCAAGGGCAGCTGGCGGTGCAGATGTCGGCC |
| Internal bar code: | CGCAGGTATATTTACGGACTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 604 |
| LEAP-Seq percent confirming: | 80.4878 |
| LEAP-Seq n confirming: | 33 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAACGCACACACACACACAC |
| Suggested primer 2: | TACCGCACGATACGTACACG |