Insertion junction: CLIP2.047206_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.047206
Chromosome:chromosome 8
Location:823560
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g361050 CNX3 (1 of 2) 4.1.99.18 - Cyclic pyranopterin phosphate synthase / Molybdenum cofactor biosynthesis protein 1; Molybdenum cofactor synthesis-step 1 protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTAGATGCAACTGAATCCAAACCCACCACCGCACTGCTCGGCTGCTGCCG
Internal bar code:TGAAGATTACGGGTAGTTTATT

Confirmation - LEAP-Seq

LEAP-Seq distance:1943
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:GATTCAGAGGAGACTGCGGG
Suggested primer 2:AGGTGTGTGTGTGTGTGTGT