Insertion cassette: | CIB2 |
Side of cassette: | 3' |
Strand: | - |
Strain: | CLIP2.047216 |
Chromosome: | chromosome 1 |
Location: | 3050476 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre01.g019300 | Nucleotidyl cyclase; (1 of 70) 4.6.1.1 - Adenylate cyclase / ATP pyrophosphate-lyase | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAAACGCGCTCTTTGGCATCAACGCCAGCGAGGCTGGCGGCGGCATCGG |
Internal bar code: | AAGATAGGTTTGGCGACAAAAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1039 |
LEAP-Seq percent confirming: | 26.3158 |
LEAP-Seq n confirming: | 5 |
LEAP-Seq n nonconfirming: | 14 |
LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AGCACCACCGTCTCATTCAG |
Suggested primer 2: | CAGGTCGTTTCTACAGGCGA |