| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.047227 |
| Chromosome: | chromosome 6 |
| Location: | 8076776 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g305251 | (1 of 1) IPR001680//IPR011045//IPR017986 - WD40 repeat // Nitrous oxide reductase, N-terminal // WD40-repeat-containing domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGAATCTCTTGATTGCGTGCTGTCGCTGCTCGCTCGGCGCCACCCGCAG |
| Internal bar code: | GCTCCACGCCAGAAAGTGGTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3791 |
| LEAP-Seq percent confirming: | 54.5455 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCAAGGATTTGGAGGAGGC |
| Suggested primer 2: | GGTCATACCGCAACACCAGA |