| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.047232 |
| Chromosome: | chromosome 3 |
| Location: | 7518285 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g205400 | (1 of 1) PF01344//PF07707 - Kelch motif (Kelch_1) // BTB And C-terminal Kelch (BACK) | intron | |
| lncRNA_TCONS_00115126 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGCACACACGATGGCGGGAATGTTGGAGGCCAGCAAGGGTTTGCCGAC |
| Internal bar code: | GGGTGATCGGCTCTTTCGCAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5388 |
| LEAP-Seq percent confirming: | 93.2692 |
| LEAP-Seq n confirming: | 97 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 104 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAATCGTATGTGGTTGGCGC |
| Suggested primer 2: | AGCTGCGTGTGTGTATTGGA |