| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.047249 |
| Chromosome: | chromosome 15 |
| Location: | 1234064 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g638551 | OPR78 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTGGGTAAGGATTTGTGGGGAAGAGTGTGTGTTAAGAGCACAACAAGA |
| Internal bar code: | TTTGGCCTTGTGCACGTTACTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 170 |
| LEAP-Seq percent confirming: | 29.4118 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTGCTGTGATGGGGTCTG |
| Suggested primer 2: | CACCTCCTCATCCCCACAAC |