Insertion junction: CLIP2.047260_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.047260
Chromosome:chromosome 12
Location:1410538
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g488752 (1 of 70) 4.6.1.1 - Adenylate cyclase / ATP pyrophosphate-lyase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGCTTGGAGGTGTGGCGTGGCCGGTGCTGGTGTTGCTGCTGCCAGGCCG
Internal bar code:TGGAAATAGTTCGATGTCCGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:1538
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CACGACTGTGCGGAAGAGAT
Suggested primer 2:GTAGAAGCTTTCCCGTGGCT