| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.047276 |
| Chromosome: | chromosome 6 |
| Location: | 8232263 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g306550 | (1 of 1) K01166 - ribonuclease T2 [EC:3.1.27.1] (E3.1.27.1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAGAAATCCGCGTTCTCCCCCATAAAGCTGGGCCATTCCAGATCCAGCG |
| Internal bar code: | GAGACGGTCTGACTTAATAAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 669 |
| LEAP-Seq percent confirming: | 23.0769 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCTGGCTCCTATACGGAC |
| Suggested primer 2: | TCGCTCAGCTGCTTGTTGTA |