Insertion junction: CLIP2.047279_3

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.047279
Chromosome:chromosome 12
Location:2850380
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g503200 (1 of 3) 3.4.24.13 - IgA-specific metalloendopeptidase / Immunoglobulin A1 proteinase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GATTGAGGCCATCAACGAGCCCGCTGCCGCCGAGACCAAGACGGAAATCG
Internal bar code:AGGGTGATTCGCAGTTATAAAC

Confirmation - LEAP-Seq

LEAP-Seq distance:2454
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:45
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:45

Suggested primers for confirmation by PCR

Suggested primer 1:CACTGCATGTTGCCTCCCTA
Suggested primer 2:ATCCCTTCTTCCTGCACTGC