| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.047282 |
| Chromosome: | chromosome 9 |
| Location: | 1272893 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g400851 | (1 of 781) IPR000104 - Antifreeze protein, type I | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTTCGCCCCCAGGTCAACCTCCTGGTCAGAACTGCCGCCGCCGCCGCCG |
| Internal bar code: | TGAGGTTAAAAGCCCAATGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 483 |
| LEAP-Seq percent confirming: | 14.2857 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTGAGCGGTCTGGATACCA |
| Suggested primer 2: | TGGTCAAAGCCATCACCTCC |