| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.047291 |
| Chromosome: | chromosome 12 |
| Location: | 2810564 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g503650 | SMM40 | S-adenosyl-L-methionine-dependent methyltransferase; (1 of 1) PTHR10108//PTHR10108:SF894 - METHYLTRANSFERASE // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGGCAGGCGGCACAGCAGGTTGGCGGCCAGGACGCAGTCCAGGCGGGAC |
| Internal bar code: | CTTTCTGACAGTTCTCTGAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 542 |
| LEAP-Seq percent confirming: | 40.0 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTTCTCCGAGTATGAGGC |
| Suggested primer 2: | TCCTCCCTTACCCCAACACA |