| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.047297 |
| Chromosome: | chromosome 3 |
| Location: | 1433070 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g151000 | SEX4,DSP8 | Laforin glucan phosphatase, chloroplastic; (1 of 1) PTHR10343//PTHR10343:SF58 - 5'-AMP-ACTIVATED PROTEIN KINASE , BETA SUBUNIT // PHOSPHOGLUCAN PHOSPHATASE DSP4, CHLOROPLASTIC | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTCGGCGCGCTCCTCCTCGGTGAGGTCGCCGCCGGGCTGCAGCAGCCGC |
| Internal bar code: | TAAGTTAGTTAACACTCGTTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1053 |
| LEAP-Seq percent confirming: | 78.2609 |
| LEAP-Seq n confirming: | 36 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGAGGGGTATGGTGGTGG |
| Suggested primer 2: | CTTGGAGTTGTCCTGCGTCT |