| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.047299 |
| Chromosome: | chromosome 4 |
| Location: | 1403520 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g214351 | Predicted protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGATGGTGCGCATGTGGGTCAGGTCAGGTCACGCCAAGCGCCAAGGGC |
| Internal bar code: | GAAATCGTGGTGTTAGGACATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2137 |
| LEAP-Seq percent confirming: | 82.6087 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGCGTGCATGACATAACAC |
| Suggested primer 2: | AGGTTCAGGTTCAGGATGCG |