| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.047334 |
| Chromosome: | chromosome 1 |
| Location: | 2508165 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g014751 | (1 of 7) PF14555 - UBA-like domain (UBA_4) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGGCGCAGATGGCAGCTCGCCGCTCGGGGTCGTCCACACCACGCACAC |
| Internal bar code: | AACCTGGCAGCGGATGTAGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4090 |
| LEAP-Seq percent confirming: | 81.5789 |
| LEAP-Seq n confirming: | 93 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 114 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACTTTTTGCCAGCAGGGAC |
| Suggested primer 2: | AGGTGGACGAGTCAGAGTCA |