| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.047338 |
| Chromosome: | chromosome 12 |
| Location: | 4630019 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g522050 | (1 of 1) PTHR11851//PTHR11851:SF140 - METALLOPROTEASE // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACGGGGTGTTGCACCGACACCTGGCCGCGCCACGCCGACAGCCCCTCGC |
| Internal bar code: | TATGGGACGCGACAGGCTGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1511 |
| LEAP-Seq percent confirming: | 56.4103 |
| LEAP-Seq n confirming: | 44 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 78 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCTAGCGCGTCCACTAGG |
| Suggested primer 2: | GACGGGGTTACGGATAGCAG |