| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.047368 |
| Chromosome: | chromosome 14 |
| Location: | 2462223 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g623900 | (1 of 41) IPR000719//IPR011009 - Protein kinase domain // Protein kinase-like domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCGAGACCGACGGGAGCCTGCGCTGCTGCGTCGGCACCGGGCCGGCGG |
| Internal bar code: | GAAAAATGCCAGTTCGATAATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4203 |
| LEAP-Seq percent confirming: | 33.3333 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTGAACAGGTGCAGGTAG |
| Suggested primer 2: | GCGCATACTCATAGGGTCCC |