| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.047369 |
| Chromosome: | chromosome 6 |
| Location: | 3318536 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g277550 | (1 of 3) 3.5.5.1 - Nitrilase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGCACAGCGACTCGTTTTATCAGAGGGCGGTGACGGCGCAGCTGGCTC |
| Internal bar code: | GTCGCGGTTAGTTATGATCCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 392 |
| LEAP-Seq percent confirming: | 44.8276 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGTGTTGAGGCAGTGTTG |
| Suggested primer 2: | CGAACAAGGTGTGCAAAGGG |