| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.047403 |
| Chromosome: | chromosome 10 |
| Location: | 6695657 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g466600 | (1 of 173) IPR029058 - Alpha/Beta hydrolase fold | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCATTAGCGCATCCTCTCGATGGTCCGGTCCTTTCCAGGCGTCCAGCCA |
| Internal bar code: | GACACGACGCGGAGTGGGTTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3309 |
| LEAP-Seq percent confirming: | 80.3571 |
| LEAP-Seq n confirming: | 90 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 112 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACAACCACTACCTGGGGC |
| Suggested primer 2: | GTGACATCGCCTCCTCTAGC |