| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.047434 |
| Chromosome: | chromosome 3 |
| Location: | 5043127 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g179961 | POLE1,POLE1-1 | (1 of 1) K02324 - DNA polymerase epsilon subunit 1 (POLE1); DNA polymerase epsilon subunit 1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGTGTTCCGCAACCTGGCCCGCTTCCACGGCTTTGGCATGCTGCGCGAG |
| Internal bar code: | TTAGCGTATTGGCGGCTTGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1091 |
| LEAP-Seq percent confirming: | 91.6667 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGCCAACAACGCACAAATC |
| Suggested primer 2: | TTCTCCCAACGAGTCATGCC |