| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.047444 |
| Chromosome: | chromosome 16 |
| Location: | 479860 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g693204 | FAP348 | Flagellar Associated Protein 348; (1 of 1) IPR001660//IPR002048//IPR013761 - Sterile alpha motif domain // EF-hand domain // Sterile alpha motif/pointed domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGGGACACGCCGCGGGCCACCCCCGATGCCGACCTCATGGCCCAGGTA |
| Internal bar code: | GAACTCTTATTCCGTAATTTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1371 |
| LEAP-Seq percent confirming: | 68.4211 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGATTTGGCCATGGAGGG |
| Suggested primer 2: | CACCACCACTACCCAATCCC |