| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.047445 |
| Chromosome: | chromosome 16 |
| Location: | 4530058 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g801958 | (1 of 6) 3.6.3.43 - Peptide-transporting ATPase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACAACCACTGGCTGCAACCTCAATCCTCATGACTGCAACCCCACACGCA |
| Internal bar code: | TCGACTCTTATCCTATGGAAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 59 |
| LEAP-Seq percent confirming: | 62.5 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAAGCCTCCTGCCCTCTCAT |
| Suggested primer 2: | GGCAGGGCATGTGTTTTGAG |