| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.047459 |
| Chromosome: | chromosome 15 |
| Location: | 911027 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g640101 | (1 of 3) IPR000104//IPR006553 - Antifreeze protein, type I // Leucine-rich repeat, cysteine-containing subtype | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGTTTGGAAGGGCGTATGTGTGTATGTGGAAGGGGCGTGGGAATGAGA |
| Internal bar code: | CCGAATGGGTCGGGTGGAGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 189 |
| LEAP-Seq percent confirming: | 20.0 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGCTGTCGTTCATTGCCG |
| Suggested primer 2: | AAACATCCGCCCATGTCCAT |