| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.047492 |
| Chromosome: | chromosome 10 |
| Location: | 3395401 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g443900 | BOR1 | (1 of 1) PF00955 - HCO3- transporter family (HCO3_cotransp); Borate transporter | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTCAAACCAGCAACACCAGAAGCTGCACTCGGGTCCCTCACCATGTCCG |
| Internal bar code: | CGGCAACCATTGGGAGTCGATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 278 |
| LEAP-Seq percent confirming: | 7.14286 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCGAAGCCATGTCAGCATT |
| Suggested primer 2: | TTTCGACTGGACCCTAACGC |