| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.047500 |
| Chromosome: | chromosome 12 |
| Location: | 1216703 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g490200 | (1 of 1) PTHR24362//PTHR24362:SF160 - SERINE/THREONINE-PROTEIN KINASE NEK // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCAGTCGCTGTCGCTGTCGCAGTCGTTATCGGAGTCGTTGTCAGAGTCG |
| Internal bar code: | GAGCTTAGTTCTGACACGTAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 259 |
| LEAP-Seq percent confirming: | 10.3448 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACTGTCACTAAGCCCAGT |
| Suggested primer 2: | CAGGACGTTGTCGGTCTTCA |