| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.047504 |
| Chromosome: | chromosome 2 |
| Location: | 5665870 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCGTAGTGCGCGCCCCGGGCGGCTTTTGCGGACTCAGCATCGCCCGCAA |
| Internal bar code: | TAGTGCATTCAACGTTGTAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1614 |
| LEAP-Seq percent confirming: | 16.6667 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 50 |
| LEAP-Seq n unique pos: | 60 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAAGCATGGTAGCAGGTCC |
| Suggested primer 2: | TAAGCCTCCGTTCGCACTTT |