Insertion junction: CLIP2.047517_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.047517
Chromosome:chromosome 3
Location:2602672
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g160300 RAM1,RAMP4 Ribosome-associated membrane protein, ER-stress-associated; (1 of 1) PF06624 - Ribosome associated membrane protein RAMP4 (RAMP4) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGCAGACCCGATCACCTGTGGGCGCATTCGCAAAGAGTCGTGTATCAGT
Internal bar code:ATATGGCGACGCTCGAACCCGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1742
LEAP-Seq percent confirming:38.2353
LEAP-Seq n confirming:13
LEAP-Seq n nonconfirming:21
LEAP-Seq n unique pos:34

Suggested primers for confirmation by PCR

Suggested primer 1:CGCTACATTTGCCGTACACG
Suggested primer 2:TTATCGCCGAGTCACCGATG