| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.047519 |
| Chromosome: | chromosome 12 |
| Location: | 2933404 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g502400 | (1 of 1) PTHR23423:SF17 - PROTEIN F40E10.6 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCCTCTCCCACCCTCGGACAGGAGACCGCCCTGGACCACACAATGCTG |
| Internal bar code: | CTCGAACGACGTCGTCGGATGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1733 |
| LEAP-Seq percent confirming: | 84.7826 |
| LEAP-Seq n confirming: | 39 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACTCACACTGCATCGCTCA |
| Suggested primer 2: | TATGCAGCGTGCCTGTAACA |