| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.047526 |
| Chromosome: | chromosome 3 |
| Location: | 8420529 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g213313 | (1 of 2) K00059 - 3-oxoacyl- (fabG) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGCCGGAGCGAGAAGCGACCTCGTGAGTCCATGGACATCTTGACTTTC |
| Internal bar code: | AATTCCAGGGTCTGAGCTGATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2269 |
| LEAP-Seq percent confirming: | 78.8462 |
| LEAP-Seq n confirming: | 41 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 52 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGAGAGGGACAGGGAGTGT |
| Suggested primer 2: | TTTGTTGCAGGTGACGCATG |