| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.047531 |
| Chromosome: | chromosome 1 |
| Location: | 4985814 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g034550 | FAP109 | (1 of 2) IPR002048//IPR003117 - EF-hand domain // cAMP-dependent protein kinase regulatory subunit, dimerization-anchoring domain; EF-Hand Containing Flagellar Associated Protein 109 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAGATGCTGCTCAGCTCCTCCGGCCCCAGCGTTTGGCCGCCGTACCGGT |
| Internal bar code: | GATCTCGATGGGAATCGGTATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3848 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 31 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACGAGGCGTATCTGGTGAG |
| Suggested primer 2: | GTTCGTCAGTGCTCCAGACA |