| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.047540 |
| Chromosome: | chromosome 7 |
| Location: | 3273792 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g334350 | (1 of 3) 2.7.1.39 - Homoserine kinase | CDS | |
| Cre07.g334400 | EXN10 | Exonuclease RNase T and DNA polymerase III; (1 of 1) PTHR30231 - DNA POLYMERASE III SUBUNIT EPSILON | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGTATGGAAGCCCGTCTTGGGCGCCGGGGCGGCCGAGGAGGACTGGGCA |
| Internal bar code: | ACACACTAGTGGGTTTCATCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 7217 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 159 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 159 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGCAGGCCTAGCACTTGAG |
| Suggested primer 2: | CGGACCTTCTCGCTCTTGTT |