Insertion junction: CLIP2.047553_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.047553
Chromosome:chromosome 9
Location:5515343
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g409050 OTU6 (1 of 2) K13719 - ubiquitin thioesterase OTU1 (OTU1, YOD1); OTU-like cysteine protease intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTTGCAGGTACCCAGCGAGGGAGCTGTGTCGGCGCTGGGACTTGCAAAC
Internal bar code:ATCACATTCAAGCGGTTCACCG

Confirmation - LEAP-Seq

LEAP-Seq distance:251
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CCAGCATCTCGTCCTCATCC
Suggested primer 2:AAGATCCTGTTCCCCAAGCG