| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.047556 |
| Chromosome: | chromosome 12 |
| Location: | 1189026 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g490450 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAACACAGGGAACGAGCTTGGCAAATACGCGCAACCCGAGCGCCTCGCGA |
| Internal bar code: | GCAACGAAGTCCCAGATGTCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4403 |
| LEAP-Seq percent confirming: | 98.4962 |
| LEAP-Seq n confirming: | 131 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 133 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTTGGCGGCATGTTCAAA |
| Suggested primer 2: | TATTGCGGCGATGCATTTCG |