| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.047564 |
| Chromosome: | chromosome 3 |
| Location: | 4176735 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g173250 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCAGCTACCAGGCCTGAGCCACACACTCCATACACGTTCCACTAGCCT |
| Internal bar code: | CGGGACCACGGACACATGCGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1413 |
| LEAP-Seq percent confirming: | 84.4444 |
| LEAP-Seq n confirming: | 38 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 45 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACAGGAGTCGTCGCGTAG |
| Suggested primer 2: | AGCACGGCACAAACAATGTC |